BDNF anticorps (Intracellular)

N° du produit ABIN7042969

Cet anticorps Cobaye Polyclonal détecte spécifiquement BDNF dans WB, IHC et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour BDNF anticorps (Intracellular) (ABIN7042969)

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivité: Humain

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp BDNF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-BDNF anticorps

Épitope: AA 166-178, Intracellular

Fonction: A Guinea Pig Polyclonal Antibody to BDNF

Specificité: Intracellular

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): The antibody is specific for BDNF, it does not crossreact with the closely related neurotrophins NGF, NT-3 or NT-4.

Homologie: Mouse,rat,many other species - identical

Attributs du produit: Guinea pig Anti-BDNF Antibody is a highly specific antibody directed against an epitope of human brain-derived neurotrophic factor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize BDNF from rat, human and mouse samples. The antibody is specific for BDNF, it does not crossreact with NGF, or . The antigen used to immunize guinea pigs is the same as Anti-BDNF Antibody (ABIN7042970, ABIN7044761 and ABIN7044762) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor)

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:500

Commentaires:

Positive Control: PCP-B250

Negative Control: (ABIN7234876)

Blocking Peptide: (ABIN7234876)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur BDNF

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Autre désignation: BDNF

Sujet: Brain-derived neurotrophic factor,Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLCγ pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system.Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both KV1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the NaV1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its "rapid" neuronal functions that include changes in neuronal excitability, plasticity and synaptic transmission.

Alternative names: BDNF, Brain-Derived Neurotrophic Factor

ID gène: 627

NCBI Accession: NM_170735

UniProt: P23560

Pathways: Signalisation RTK, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity